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胸腺肽纯化工艺的改进

李光谱,迟春萍,张秀霞,关晓峰,王志武,盛军,郭岩

CHINESE JOURNAL OF BIOLOGICALS（2003）

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Abstract
目的建立高效、快速、简便的纯化胸腺肽方法,以便提高胸腺素α1含量及活性,减少过敏反应.方法采用DEAE Sepharose FF、CM-Sephrose两种串联柱层析纯化.结果纯化后的胸腺肽电泳结果出现明显的胸腺素α1带、玫瑰花结率可达33%(绝对值)以上,相对分子质量明显在3 100左右,无过敏反应.结论建立了高效、规模化的胸腺肽纯化方法,提高了胸腺肽质量.
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