碱预处理玉米芯米根霉同步糖化发酵产富马酸

Chinese Journal of Bioprocess Engineering(2015)

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Abstract
以碱预处理玉米芯渣为原料,采用单因素优化方法优化米根霉同步糖化发酵产富马酸。在此基础上,研究米根霉利用碱预处理玉米芯渣的同步糖化发酵,并与纯糖发酵进行对比。结果表明:在50 g/L底物、( NH4)2 SO4质量浓度0?71 g/L、纤维素酶用量20 FPIU(以1 g纤维素计)、CaCO3加入量30 g/L、接种量10%(体积分数)和装液量50 mL的条件下,米根霉同步糖化发酵过程产富马酸13?78 g/L,而纯糖发酵富马酸生成量仅6?21 g/L。
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Key words
alkali-pretreated,corncob,fumaric acid,simultaneous saccharification and fermentation,Rhizopus oryzae
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