漆酶高产工程菌构建及漆酶对RBBR的脱色作用
CHINESE JOURNAL OF BIOPROCESS ENGINEERING（2004）
Abstract
研究提取产漆酶白腐菌(Fomelignosus)的总RNA,利用RT-PCR克隆到漆酶的cDNA,并将其克隆到表达载体pGAPZA,重组质粒经线性化、电激转化Pichia pastoris GS115、通过底物显色反应筛选漆酶生产工程菌株,在最适培养条件下该菌株产酶活力高达9.03U·mL-1.纯化得到漆酶对RBBR(Remazol brilliant blue R)有很好的脱色作用,该酶的最适脱色pH为5.0,最适脱色温度为30℃.当溶液中漆酶活力为1.0 U·mL-1,在最适脱色条件下作用12 h,100mg·L-1RBBR溶液脱色率可达90%以上.
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