细胞型朊蛋白抑菌活性的初步探索
Letters in Biotechnology(2016)
Abstract
目的:研究细胞型朊蛋白(PrPc)有无抑菌活性.方法:把prp分别与金黄色葡萄球菌、枯草芽孢杆菌、巨大芽孢杆菌、苏云金芽孢杆菌、大肠杆菌等5种菌孵育一定时间,然后用碘化丙锭对上述5种样品染色后采用流式细胞仪检测各样品的荧光信号.结果:通过对流武细胞仪的荧光信号进行分析,发现PrPc对金黄色葡萄球菌和枯草芽孢杆菌具有明显的抑制活性,而另外3种菌的样品没有检测到荧光信号的增强,亦即PrPc对这3种菌无抑菌活性.结论:细胞型朊蛋白有一定的抑菌活性,有望成为一种新型的抗菌肽.
MoreKey words
cellular prion protein,antibacterial activity,antimicrobial peptides,fluorescence signal
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