发根农杆菌诱导玉米毛状根发生及再生植株
SCIENCE IN CHINA(SERIES C)(2005)
Abstract
发根农杆菌(Agrobacterium rhizogenes)侵染玉米愈伤组织15天左右获得毛状根. 转化根在不含激素的培养基上快速生长, 并表现出典型的毛状根性状. 在含ZT 1.6 mg/L和NAA 0.4 mg/L的MS培养基上, 毛状根再生成完整转化植株. PCR-Southern杂交证实T-DNA已整合入转化株的染色体组.
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