PBMC中HBV cccDNA与HBV宫内感染关系的研究
MODERN PREVENTIVE MEDICINE（2005）
Abstract
目的:对外周血单个核细胞(peripheral blood mononuclear cells,PBMC)中乙型肝炎病毒(HBV)共价闭合环状DNA(covalently closed circular DNA,ccc DNA)进行扩增,并对其产物直接测序鉴定,以证实PBMC为HBV肝外复制的场所,从而进一步证实PBMC感染为乙型肝炎宫内感染的途径之一.方法:选择性PCR扩增PBMc中HBV cccDNA,并对1例阳性产物直接测序鉴定,测序结果与GeneBank中已知序列进行同源性比较.结果:HBsAg阳性孕妇PBMC HBV cccDNA的检出率为9.93%,新生儿PBMC HBV ccc DNA的检出率为4.58%,PBMC HBV cccDNA与公布的已知序列的同源性为100%.结论:PBMC为HBV肝外复制的场所,PBMC感染很可能是乙肝宫内感染的传播机制之一.
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