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RP-HPLC测定人参果总皂苷中人参皂苷Re和人参皂苷Rd的含量

曲芯瑶,赵宏峰,弥宏,庞威,郭禹彤,金光洙

Chinese Community Doctors（2014）

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Abstract
目的:建立高效液相色谱法测定人参果总皂苷中人参皂苷Re和人参皂苷Rd含量的方法.方法:采用Apollo C18色谱柱(250mm×4.6mm,5μm),乙腈-水梯度洗脱,流速1.0ml/分,检测波长203nm,柱温25℃.结果:人参皂苷Re、人参皂苷Rd在70分钟内达基线分离,线性关系良好(r≥0.9990).加样回收率分别为98.5％和97.8％.结论:该方法简便、快速,可作为人参果总皂苷的质量控制指标.
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