高效液相色谱法测定泡菜中姜黄素
wf(2016)
Abstract
本实验建立了二极管阵列检测器测定泡菜中姜黄素的高效液相色谱法。实验中采用XDB-C8柱(150 mm×4.6 mm,5μm)分离,以4%乙酸-甲醇为流动相,梯度洗脱,柱温35℃,检测波长为430 nm,整个分离过程在12 min内完成。该方法加标回收率范围:93.0%~98.0%,方法检出限:1 mg/kg。
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