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人微血管内皮细胞不同浓度的siRNA转染效果的实验观察

Bao Jian Wen Hui(2017)

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Abstract
目的:观察不同浓度siRNA对人微血管内皮细胞HMEC-1细胞转染效果的影响,优化HMEC-1细胞的转染方法和siRNA用量.方法:HMEC-1细胞采用相同的细胞量接种处理后,荧光标记的siRNA (FAM-siRNA)随机分为高中低浓度及对照四个组,然后运用Lipofectamine@2000 Reagent转染试剂进行转染,培养48h后荧光显微镜观察转染的效果.结果:中高浓度siRNA转染效果较低浓度组好,且中高浓度转染效果相同.结论:中浓度siRNA更适合HMEC-1细胞的转染.
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