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1型胰岛素样生长因子受体(IGF-1R)重组单链可变片段的制备及表达

Home Medicine(2019)

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Abstract
实验设计(DoE)是一种基于统计的实验设计技术,可以克服传统的单因素一次法(OFAT)的蛋白质纯化优化方法的缺点.本研究采用 DoE方法对大肠杆菌中表达的1型胰岛素样生长因子受体(ⅡGF-1R)进行重组单链可变片段(scFv)的纯化.
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