重组人表皮生长因子融合蛋白发酵条件优化
Special Health(2018)
Abstract
目的:优化GST-hEGF融合蛋白工程菌pGEX-EGF/BL21STAR(DE3)pLysS30L发酵罐培养及表达条件.方法:上罐发酵采用M9-YT-Glu发酵培养基,IPTG诱导;通过还原SDS-PAGE法测定目标蛋白表达量、落计数法测定质粒丢失率,以确定表达温度、时间以及IPTG添加量等参数.结果与讨论:hEGF重组菌诱导温度为30℃、加入终浓度0.2M IPTG诱导,维持诱导时间4hr,目的蛋白表达量占菌体蛋白总量的30%左右,为后续rEGF融合蛋白纯化奠定了基础.
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