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血清miR-499a-3p在不稳定型心绞痛中的表达

Special Health(2018)

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Abstract
目的:检测miR-499a-3p在不稳定型心绞痛(Unstable angina,UA)与疑似心绞痛非冠心病患者血清中的表达差异.方法:收集实验组UA患者(n=30)及对照组疑似心绞痛的非冠心病患者(n=21)外周血.利用HiPure Serum/Plasma miRNA Kit分离方法,获取血清中总RNA.Real-Time PCR检测两组血清miR-499a-3p表达量.结果:通过相对定量分析2-△△Ct法算出两组miR-499a-3p的相对表达量,使用SPSS统计学软件分析,与对照组相比较,UA组血清miR-499a-3p的表达量明显升高(P<0.05).结论:实验组较对照组血清miR-499a-3p的表达水平增高,可为UA的有效检测指标提供新的策略.
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