HPLC法测定参味补肾口服液中淫羊藿苷的含量

SHAANXI JOURNAL OF TRADITIONAL CHINESE MEDICINE（2003）

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Abstract

目的:建立用HPLC法测定参味补肾口服液中淫羊藿苷的含量的方法.方法:色谱柱为C18柱,乙腈-水(30:70)为流动相;检测波长270nm,理论塔板数按淫羊藿苷峰计算应不低于1500.结果:淫羊藿苷在0.2008～1.0040μg范围内呈线性关系,γ=0.9996;平均回收率为97.8%,RSD=1.2%(n=6).结论:该方法简便准确重现性好,灵敏度高,可用于测定参味补肾口服液中淫羊藿苷的含量.

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