RP-HPLC法测定小果博落回中7种异喹琳类生物碱
CHINESE TRADITIONAL AND HERBAL DRUGS(2010)
Abstract
目的 建立同时测定小果博落回中7种生物碱的高效液相色谱法.方法 采用高效液相色谱法,色谱柱为WatersXBridge C18柱(250 mm×4.6 mm,5μm),流动相:(A)乙腈,(B)0.5%三乙胺缓冲液(磷酸调pH=2.5),梯度洗脱,体积流量0.8 mL/min,进样量5μL,柱温3 5℃,检测波长284 nm.结果 7种生物碱均能达到良好的分离,原阿片碱、别隐品碱、血根碱、小檗碱、白屈菜红碱、二氢白屈菜红碱、二氢血根碱的线性范围分别为0.11~22.0,0.424~84.7,0.433~86.6,0.380~75.9,0.498~99.6,0.523~104.5,0.508~101.5 μg/mL;平均回收率分别为97.8%,97.0%,98.9%,102.2%,98.6%,101.8%,102.9%.结论 本方法操作简便、准确、灵敏度高,为该类药材资源的质量评价提供了一定的科学依据.
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