Taraffinisoside A对HepG2.2.15细胞HBsAg、HBeAg及HBV-DNA表达的影响
Chinese Traditional Patent Medicine(2015)
Abstract
目的 研究六月青中一种苯乙醇苷taraffinisoside A的体外抗乙肝病毒作用.方法 以HepG2.2.15细胞株为模型,通过MTT法检测taraffinisoside A对HepG2.2.15细胞的细胞毒作用.采用酶联免疫吸附法(ELISA)检测细胞上清液乙肝表面抗原(HBsAg)和e抗原(HBeAg)表达水平,实时荧光定量PCR法检测细胞上清液HBV DNA的含有量.结果 Taraffinisoside A对细胞分泌HBsAg、HBeAg和HBV DNA具有明显的抑制作用(P<0.01,P<0.05).抑制作用随药物浓度的增加而逐渐增强.结论 Taraffinisoside A在体外有明显的抗乙肝病毒作用.
MoreKey words
Liuyueqing (Tarphochlamys affinis [Griff]),taraffinisoside A,HBV
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