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黄芪注射液对贫血小鼠脂肪细胞分泌肿瘤坏死因子的影响

祝晓玲,祝彼得,黄秀深,杨代耘,易静,杨岚,周毅

JOURNAL OF CHENGDU UNIVERSITY OF TRADITIONAL CHINESE MEDICINE（2002）

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Abstract
目的观察黄芪注射液(AMI)对贫血小鼠脂肪细胞分泌肿瘤坏死因子(tumor necrosis factor, TNF)的影响.方法在贫血小鼠脂肪细胞培养体系中,分别加入0.1 mL不同浓度的AMI(终浓度分别为40 μg/mL、400 μg/mL)作为药物组1、药物组2,对照组加入等量RPMI-1640培养液,分别收集培养上清液,采用TNF敏感细胞株L929细胞,以MTT比色法检测各实验组TNF活性单位.结果与对照组比较,各药物组脂肪细胞培养上清所含TNF均有明显升高.结论一定浓度的AMI体外作用,可以刺激贫血小鼠脂肪细胞分泌TNF.
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