附子含药血清对大鼠原代肝细胞能量代谢的影响

Dongfang KAN,Xuming JI,Qian CHEN,Wanchen YU,Jibiao WU

Liaoning Journal of Traditional Chinese Medicine（2018）

Cited 1|Views6

No score

Abstract

目的:探讨附子含药血清对大鼠原代肝细胞能量代谢的影响.方法:制备含药血清,大鼠原代肝细胞随机分为空白血清组和含药血清组.干预24 h后,分光光度计检测细胞内Na+-K+-ATP酶、Ca2+-Mg2-ATP酶活性,高效液相色谱法(HPLC)检测细胞内ATP、ADP、AMP含量及细胞能荷,激光共聚焦显微镜检测线粒体膜电位.结果:与空白血清相比,附子含药血清可显著升高肝细胞Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性,增加细胞内ATP含量,升高线粒体膜电位.结论:热性中药附子促进细胞能量代谢,增加细胞内能量储备.

AI Read Science

Must-Reading Tree

Example

Generate MRT to find the research sequence of this paper

Chat Paper

Summary is being generated by the instructions you defined