电针结合吗啡对骨癌痛大鼠痛觉敏化及脊髓星形胶质细胞的活化作用
Shanghai Journal of Traditional Chinese Medicine(2016)
Abstract
目的 观察电针结合吗啡对骨癌痛大鼠痛觉敏化及脊髓星形胶质细胞的活化作用.方法 将60只雌性Wistar大鼠随机分为5组,每组12只.模型组、吗啡组、电针组和电针吗啡组大鼠右侧胫骨注入Walker 256大鼠乳腺癌细胞制备骨癌痛模型,假手术组大鼠注入Hanks液作为对照.造模后第15 d起,分别予以相应的电针和(或)吗啡干预,每天1次,连续6天.治疗前后,检测大鼠热刺激缩爪潜伏期(PWL),并采用Western blot法和免疫荧光组织化学法分别测定脊髓背角胶质原纤维酸性蛋白(GFAP)、白细胞介素1β(IL-1β)和神经生长因子(NGF)的表达情况.结果 ①吗啡组、电针组和电针吗啡组大鼠的PWL均高于模型组(P<0.01),电针吗啡组PWL高于吗啡组与电针组(P<0.01).②与模型组比较,电针组和电针吗啡组脊髓GFAP和NGF表达显著降低(P<0.01);吗啡组、电针组和电针吗啡组脊髓IL-1β表达显著降低(P<0.01).结论 电针结合吗啡可抑制骨癌痛大鼠痛觉敏化,其镇痛机制是能抑制脊髓背角星形胶质细胞的活化.
MoreKey words
electroacupuncture,acupuncture with medicine,cancer pain,acupuncture analgesia,astrocyte
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