基于遗传算法的土香薷总黄酮提取工艺优化研究

Journal of Zhejiang Chinese Medical University(2014)

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Abstract
[目的]确定土香薷总黄酮的最优提取工艺。[方法]以乙醇浓度、料液比、提取时间和提取次数为考察因素,以总黄酮含量为目标函数,运用SPSS18.0软件建立其正交试验数据的二次回归模型,并进一步采用遗传算法对其进行优化,确定土香薷总黄酮的最优工艺条件。[结果]建立总黄酮含量(Y)与各因素的二次回归模型为:Y=51.039+2.164X3X4-0.007X1X2;遗传算法优化后得其最优提取工艺条件为:加药材21倍量浓度为61%的乙醇提取3次,每次2h。;此条件下土香薷总黄酮的平均含量为54.37 mg/g,RSD为1.29%,与预测值(53.71mg/g)基本相近。[结论]所选最优提取条件提取率高、稳定性好,可用于土香薷总黄酮的提取。
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Key words
genetic algorithm,orthogonal design,Origanum vulgare Linn,total flavonoids,process optimization
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