延胡索药材及饮片的UPLC质量控制方法
Journal of Tianjin University of Traditional Chinese Medicine(2015)
Abstract
[目的]检测延胡索药材及饮片中有效成分的含量,建立延胡索药材和饮片的超高液相色谱法(UPLC)质量控制方法.[方法]采用UPLC检测方法,ACQUITY UPLC BEH C1s色谱柱(100 mm×2.1 mm,1.7 μm),乙腈(B)-0.1%甲酸水溶液,调pH值至5.0(A),流速:0.3 mL/min,柱温:45 ℃,进样量:5μL;梯度洗脱.以延胡索乙素和去氢紫堇碱的含量为指标对延胡索的水提液进行检测.[结果]样品中延胡索乙素的含量在0.229 1~0.775 5 mg/g之间,去氢紫堇碱的含量在0.781 0~2.931 0 mg/g之间,不同样品延胡索乙素和去氢紫堇碱的含量差异很大.[结论]UPLC法的灵敏度更高、检测速度更快,适用于组分多、成分复杂的中药材的分析及质量的评价.
MoreKey words
Corydalis Rhazamo,UPLC,tetrahydropalmatine,dehydrocorydaline
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