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HPLC测定大解毒颗粒中盐酸小檗碱的含量

王梅,吴凌,王林凤,刘航

JOURNAL OF SHAANXI COLLEGE OF TRADITIONAL CHINESE MEDICINE（2009）

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Abstract
目的 建立大解毒颗粒中盐酸小檗碱的HPLC测定方法.方法 采用Sphevisorb-C18(200mm×4.6mm,5μm)色谱柱,检测波长265 nm.结幂色谱条件为:以乙腈-0.1%磷酸溶液(50:50)(每100mL加十二烷基硫酸钠0.05 g),流速为1.0 mL/min.本方法线性范围为0.084 μg～0.56 μg,r=0.9999;方法精密度RSD为0.4%(n=6);平均回收为98.40%,RSD为1.02%(n=6).结论 该方法准确度高,重现性良好,可作为大解毒颗粒中盐酸小檗碱的含量测定方法及其定量控制指标.
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