LC-MS/MS同时测定配伍知母对黄柏中5种成分含量的影响
Journal of Chinese Medicinal Materials(2017)
Abstract
目的:建立LC-MS/MS法同时测定黄柏中巴马汀、药根碱、黄柏碱、木兰花碱和小檗碱的含量,研究配伍知母对黄柏中5种成分含量的影响.方法:采用ZORBAX Eclipse XDB-C18色谱柱(150 mm×2.1 mm,3.5 μm),流动相为0.1%甲酸溶液-乙腈(65:35),流速0.25 mL/min,柱温30℃;质谱条件为电喷雾(ESI)离子源,正离子多反应离子监测(MRM)用于定量测定.结果:巴马汀、药根碱、黄柏碱、木兰花碱和小檗碱分别在3.42~91.2 ng/mL、3.15~ 84.0 ng/mL、3.45 ~ 92.0 ng/mL、2.97 ~ 79.2 ng/mL、179.0 ~1 253.0 ng/mL浓度范围内线性关系良好(r≥0.9994);平均加样回收率均在99.5%~102.0%.黄柏配伍知母后,除药根碱外其他4种成分含量均有变化.结论:该方法快速、准确、重复性好,可用于黄柏中5种成分的含量测定;配伍知母对黄柏中巴马汀、木兰花碱、黄柏碱和小檗碱的含量有影响.
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