HPLC同时测定栀子药材中两类活性成分
World Science and Technology-Modernization of Traditional Chinese Medicine(2015)
Abstract
目的:本文建立了同时测定栀子药材中有机酸类(新绿原酸、绿原酸、隐绿原酸等)、环烯醚萜苷类(京尼平苷酸、去乙酰车叶草酸甲酯、京尼平龙胆双糖苷、栀子苷等)2类有效成分的高效液相色谱含量测定方法,用于栀子药材的质量控制.方法:采用Kromasil C18(250× 4.6 mm,5μm);流动相:乙腈(A)-0.1%磷酸溶液(B),梯度洗脱,0-10 min,5%→10%(A);10-20 min,10%→18%(A);20-30 min,18%→20%(A);流速1 mL·min-1,进样量10μL,柱温35℃,检测波长为324 nm和238 nm.结果:20 min内绿原酸、绿原酸、隐绿原酸、京尼平苷酸、去乙酰车叶草酸甲酯、京尼平龙胆双糖苷、栀子苷等被测组分均出峰且均达到基线分离;在一定浓度范围内具有良好的线性关系,新绿原酸:Y=24.62X-0.462(r=0.999 9);绿原酸:Y=27.76X -3.045(r=0.999 9);隐绿原酸:Y=24.07X-0.569(r=0.999 8);京尼平苷酸:Y=12.95X -1.088 (r=1.000 0);去乙酰车叶草酸甲酯:Y=14.57X-0.693(r=0.999 8);京尼平龙胆双糖苷:Y=7.261X -0.117 (r=0.999 9);栀子苷:Y=13.60X-13.59(r=0.999 9),平均回收率均在95%-105%.结论:该检测方法快速、准确、灵敏,可为栀子药材多成分质量控制提供了一定的参考.
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Key words
HPLC,Gardenia jasminoides Ellis,multiple components determination,quality control
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