HPLC测定天麻配方颗粒中天麻素和对羟基苯甲醇的含量
Modern Chinese Medicine(2018)
Abstract
目的:建立天麻配方颗粒中天麻素和对羟基苯甲醇含量测定方法,为其质量控制提供可靠依据.方法:采用高效液相色谱法,Welch Ultimate LP-C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.1%磷酸(4:96)为流动相,检测波长为220 nm,流速为0.8 mL·min-1,柱温为30℃.结果:天麻素在49.31~986.2μg·mL-1范围内呈良好线性(r=0.9996),加样回收率平均值为102.68%,RSD值为1.66%;对羟基苯甲醇在7.589~151.78μg·mL-1范围内呈良好线性(r=0.9996),加样回收率平均值为97.88%,RSD值为1.96%.结论:该方法精密度、重复性和分离效果好,分析快速、准确,适用于对天麻中天麻素和对羟基苯甲醇的定性、定量研究.
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