HPLC测定北京妙峰山玫瑰花中芦丁的含量

Modern Chinese Medicine(2017)

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Abstract
目的:采用高效液相色谱法测定北京妙峰山玫瑰花中芦丁的含量.方法:超声提取法,色谱柱为Agilent zorbax SB-C18(250 mm×4.6 mm,5μm),检测波长为310 nm,柱温为25℃,流动相为0.2%冰醋酸-乙腈(90:10),流速为0.8 mL,min-1.结果:北京妙峰山玫瑰花中芦丁的含量为1.2549 mg·g-1.结论:该方法简便、快速、准确、重复性好,适用于玫瑰花中芦丁的含量测定.
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Key words
HPLC,rutin,rose flowers,Miaofeng Mountain
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