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脾虚证大鼠模型胃组织cAMP-PKA信号传导通路变化的研究

宋囡,贾连群,王俊岩,单德红,何丽娟,曹媛,朱美林,杨关林

Chinese Journal of Basic Medicine in Traditional Chinese Medicine（2015）

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Abstract
目的:探讨脾气虚证和脾阳虚证大鼠胃组织的改变与cAMP-PKA信号通路的相关性.方法:将24只雄性SD大鼠按随机数字表法分为正常组、脾气虚组、脾阳虚组,脾气虚采用饱食1d、禁食2d、游泳15 d,脾阳虚采用脾气虚证加灌服番泻叶造模,造模后检测大鼠胃组织cAMP活性及PKA、CREB mRNA和蛋白的表达.结果:脾气虚组和脾阳虚组胃组织cAMP活性及PKA、CREB mRNA和蛋白表达均减弱.结论:脾气虚证和脾阳虚证大鼠胃组织的改变与cAMP-PKA信号传导通路有一定的相关性.
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Key words
Spleen Qi deficiency syndrome,Spleen Yang deficiency syndrome,cAMP/PKA,Signal transduction
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