HPLC法同时测定产康乐合剂中3种成分的含量
Guiding Journal of Traditional Chinese Medicine and Pharmacology(2017)
Abstract
目的:建立产康乐合剂中三种有效成分含量测定方法.方法:Agilent TC-C18色谱柱(4.6 mm×250mnm,5μm),以0.2%磷酸水溶液和乙腈为流动相(梯度洗脱),进样量10 μL,柱温25℃,检测波长为325 nm.结果:绿原酸、黄芩苷、黄芩素分别在7.12~71.18 μg/mL;29.39~293.89 μg/mL和2.12~21.17 μg/mL范围内线性关系良好,平均加样回收率分别为102.61%、103.21%和97.76%(RSD%值分别为1.77%、0.77%和1.48%).重复性、稳定性、精密度等RSD%均小于2%.结论:该方法简单易行,可用于产康乐合剂质量控制.
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