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HPLC法测定心脉乐片中丹参酮ⅡA的含量

Guiding Journal of Traditional Chinese Medicine and Pharmacology(2012)

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Abstract
目的:建立心脉乐片中丹参酮ⅡA的含量测定方法.方法:采用HPLC法,色谱柱:Kromasi C18(4.6 mm×250 mm,5μm);流动相:甲醇-水(75:25);检测波长:270 nm;柱温:室温;进样量:10 mL.结果:丹参酮ⅡA回归方程为Y=173.7X+2817835,在0.06-0.14 μg范围内与峰面积具有良好的线性关系,r=0.9999;平均加样回收率为97.50%,RSD%为0.71%(n=6).结论:该法简便、准确,灵敏度高,重现性好,可作为控制心脉乐片的质量控制方法.
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