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高效液相色谱法测定四味光明盐汤散中没食子酸的含量

Wen-yan SHI,Bao-feng GUO, Yu-hua WANG

Research and Practice on Chinese Medicines（2015）

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Abstract
目的 建立四味光明盐汤散中没食子酸的含量测定方法.方法 采用高效液相色谱法.色谱柱为Phenomen C18柱(250mm×4.6mm,5μm);流动相为甲醇-0.1％磷酸(7∶93);检测波长为272nm;流速为1.0ml·min-1;柱温30℃.结果 没食子酸在60.00～600.0 ng范围内与峰面积积分值呈良好的线性关系(γ =1.000 0),回归方程为Y=13 642X+58 170,平均回收率为99.0％,RSD为1.51％(n=9).结论 该方法准确、精密、重复性好,可作为四味光明盐汤散中没食子酸的含量测定方法.
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Key words
Siwei Guangmingyan Tangsan,gallic acid,HPLC
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