星点设计—效应面法优化苦参方的醇提工艺
Research and Practice on Chinese Medicines(2015)
Abstract
目的 优化苦参方的乙醇提取工艺.方法 以乙醇浓度、溶剂倍量、提取时间为自变量,以K(苦参碱与氧化苦参碱总和)提取率及干膏得率的总评“归一值”为因变量,通过对自变量各水平进行多元线性回归及二项式拟合,选择合适的拟合方程模型,采用效应面法优选最佳工艺,并进行预测分析.结果 最优提取工艺条件为8倍量65%乙醇,提取2次,每次2.5 h,总评“归一值”实测值与预测性偏差为-2.26%,二项式拟合复相关系数R2=0.949 9.结论 星点设计-效应面法优选的提取工艺稳定可行,预测性良好.
MoreKey words
Sophora flavescens,central composite design,response surface methodology,matrine,oxymatrine,alcohol extraction process
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