柠檬苦素调控Tiam1基因表达影响人体血管瘤内皮细胞VEGF/VEGFR2通路及诱导其凋亡的机制研究
Chinese Journal of Immunology（2020）
Abstract
目的:研究柠檬苦素Toonaciliatin A对血管瘤内皮细胞(HemEC)增殖、凋亡的影响及潜在作用机制.方法:胶原蛋白酶A分离HemEC细胞,MTT法测定不同浓度Toonaciliatin A对HemEC细胞生存率的影响,流式细胞术测定HemEC细胞凋亡率,Western blot检测蛋白表达,qRT-PCR检测RNA的表达.结果:Toonaciliatin A能够抑制HemEC增殖,促进HemEC细胞凋亡;Toonaciliatin A抑制Tiam1蛋白表达,过表达Tiam1逆转了Toonaciliatin A对HemEC的抑制作用;Toonaciliatin A能够抑制VEGF和VEGFR2蛋白表达,过表达Tiam1逆转了Toonaciliatin A对VEGF和VEGFR2蛋白表达的抑制作用.结论:柠檬苦素类似物Toonaciliatin A通过靶向Tiam1基因抑制VEGF/VEGFR2通路抑制HemEC细胞增殖,促进细胞凋亡.
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