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戊型肝炎病毒Ch-S-1株全长体外转录载体的构建及表达初步研究

朱光泽,李一权,兰添,范园园, 张诺娜,胡宁宁,邢彬,尹秀英,李霄

Chinese Journal of Immunology（2015）

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Abstract
目的:构建出HEV Ch-S-1株的全长cDNA克隆,并转染至HepG2细胞并对其表达进行初步研究. 方法:利用重叠PCR方法获得HEV Ch-S-1株全长PCR产物;构建体外转录载体pKS-HEV,成功获得HEV基因组RNA,并转染至HepG2细胞,通过RT-nPCR、套式PCR和间接免疫荧光实验检测病毒的表达. 结果:成功构建了HEV基因组全长cDNA体外转录载体pKS-HEV,并证明了该cDNA克隆在HepG2细胞中成功进行了表达. 结论:成功构建了HEV Ch-S-1株全长体外转录载体pKS-HEV,并对其表达进行了初步研究,为将来在分子水平上研究HEV及研发疫苗提供了一些实验基础.
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Key words
Hepatitis E virus,Reverse genetics,Full-length cDNA clone,Expression vector
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