蜕皮激素诱导MIIP基因的表达对肝癌细胞增殖迁移的影响
Progress in Modern Biomedicine（2017）
Abstract
目的:探讨蜕皮激素诱导迁移侵袭抑制蛋白基因(migration and invasion inhibitory protein,MⅡP)基因的表达对肝癌细胞SK-Hep-l增殖和迁移能力的影响.方法:采用PCR扩增MIIP基因,连入T载体测序正确后,插入到蜕皮激素可诱导真核表达载体pIND中.将pIND-MHP和含蜕皮激素受体基因的表达载体pVgRXR以脂质体转染法转染到SK-Hep-1中,经G418和Zeocin 双抗生素筛选获得稳定转染细胞株.蜕皮激素PonasteroneA诱导后,采用Western blot检测MIIP表达,CCK-8实验检测细胞增殖,划痕实验检测细胞迁移.结果:蜕皮激素诱导MIIP表达上调后,SK-Hep-1细胞的增殖能力显著下降(P＜0.05),细胞的迁移能力明显减弱.结论:建立了MIIP基因可诱导表达系统,证实在肝癌细胞SK-Hep-1中MIIP过表达可抑制细胞的增殖及迁移能力.
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Key words
Hepatic carcinoma,MIIP,Inducible gene expression,Migration and invasion
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