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陕西株利什曼原虫ITS-1基因片段序列多态性分析

Dong-li LIU,Feng-ping WANG,An-li WANG,Zheng ZHANG,Tian-hai WANG,Yi SHI,Rong-hui GUAN,Wen-juan LI

Journal of Parasitic Biology（2018）

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Abstract
目的 分析陕西株利什曼原虫rRNA内转录间隔区间I(ITS-1)序列多态性,探讨利什曼原虫ITS-1分子遗传规律. 方法 陕西省韩城市犬、白蛉以及人体利什曼原虫阳性标本7份,提取核酸,PCR法扩增ITS-1片段,双向测序、拼接后与文献发表的代表株进行序列比对,构建系统发育树,进行系统发育分析. 结果 7份标本均扩增出约320 bp的片段,ITS-1序列之间同源性＞99％,与国内山丘型疫区虫株高度一致,与荒漠型遗传距离较远.系统发育分析陕西株与婴儿利什曼原虫和杜氏利什曼原虫聚为一类. 结论 陕西省韩城市分离自病犬、传播媒介白蛉及黑热病患者的利什曼原虫ITS-1序列同源,应为犬源型婴儿利什曼原虫.
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Key words
Anthrax,TaqMan-LNA probes,multiplex real-time quantitative PCR,locked nucleic acids(LNAs),prophage
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