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CRISPR系统中Cas蛋白的分类及作用机制

Huan-huan FENG,Cai-long SHAN,Jin-yue LI, Jia-jing LU, Xi-lu WANG,Qin-hao SHEN,Hong-mei JIAO,Yin-yan YIN,Gui-mei KONG,Ting-ting GUO,Guo-cai LI

Journal of Parasitic Biology（2018）

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Abstract
CRISPR(clustered regularly interspaced short palindromic repeats)系统存在于40％细菌与90％古细菌中,由前导序列、回文重复序列、相关Cas蛋白三部分构成.Cas蛋白是CRISPR系统中的-类核酸酶,对CRISPR系统发挥获得性免疫功能具有重要作用.已鉴定出至少45个Cas蛋白家族,其多样性对现有分类系统提出了巨大挑战.不同类型的Cas蛋白在CRISPR系统适应、表达、干扰等作用阶段发挥的功能各异.本文主要讨论Cas蛋白的分类及作用机制的研究进展.
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