人胎盘绒毛膜间充质干细胞分离培养的新方法

Shusheng TANG,Xunsha SUN,Jieying WU,Yan LU,Jinsong CHEN,Fatao LI,Jie TANG,Shaoqing WU

Journal of Biomedical Engineering Research（2018）

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Abstract

建立一种简便、快捷的人胎盘绒毛膜间充质干细胞(human placental chorionic -derived mesenchy-mal stem cells, hpcMSCs)分离培养方法.用手持电动匀浆器处理人绒毛膜,经酶消化后接种培养,用倒置显微镜观察细胞形态,CCK-8测定细胞生长曲线,流式细胞术检测细胞表面标记,成脂、成骨诱导分化试剂盒鉴定细胞的分化潜能.获得的人胎盘绒毛膜间充质干细胞为成纤维细胞样细胞,呈平行排列或漩涡状生长,高表达CD73、CD105、CD90,而CD34、CD45、CD14、CD19、HLA-DR呈阴性,生长曲线为" S"型,经成骨或成脂诱导后,茜素红染色或油红O染色呈阳性.符合国际细胞治疗学会认可的间充质干细胞标准.建立了人胎盘绒毛膜间充质干细胞的分离培养新方法,结合了组织块法和酶消化法的优点,为绒毛膜间充质干细胞的应用提供基础.

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Key words

Mesechymal stem cells,Chorion,Cell isolation,Cell cultivation,Method

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