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STR和SNP对3个小鼠1号染色体替换系筛选结果的比较

Li-ya ZHAO,Ying ZHAO,Tian-zhu CHAO,Lei LIU,Zheng-hong XING,Guo-qiang CHEN,Jun-hua XIAO

Laboratory Animal and Comparative Medicine（2012）

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Abstract
目的 比较STR和SNP对3个1号染色体替换系的分型结果,并为每个替换系选择合适的基因分型方法.方法 采用20个SNP和10个STR位点分别对3个1号染色体替换系N2代各40个样本进行分型.结果 采用20个SNP位点,从C3H/He替换系中筛选到6个样本,从FVB/N替换系中筛选到9个样本,从AKR替换系中筛选到4个样本.采用10个STR位点,从C3H/He替换系中筛选到6个样本,从FVB/N替换系中筛选到10个样本,从AKR替换系中筛选到6个样本.结论 根据每个品系基因组背景不同,可选择三组多重STR方案作为C3H/He替换系的基因分型方法,三组多重SNP方案作为FVB/N和AKR替换系的基因分型方法.
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