Chrome Extension
WeChat Mini Program
Use on ChatGLM
VIPCreated with Sketch.

Spycatcher作为一种新的高效促溶标签蛋白的探讨

Kun WANG,Ri-fei LI,Wei-guo HE,Yuan-jie XIE,Jin-feng SHI,Si-jie TAN,Zhong-cheng MO,Mei-xiang LI

Progress of Anatomical Sciences（2017）

Cited 0|Views4
No score
Abstract
目的 探究spycatcher可作为一种新的高效促溶标签蛋白.方法 通过分子生物学方法将spycatcher基因与包涵体PD1及MPD1基因融合构建到表达载体PET21a中,然后通过ELISA和fortebio验证其融合蛋白活性.结果 spycatcher与包涵体靶蛋白PD1及MPD1融合后为可溶性表达,融合蛋白纯化后能够与其配体PDL1有效结合.结论 Spycatcher可作为一种新的高效促溶标签蛋白,这将为不可溶表达蛋白的表达纯化带来诸多便利.
More
Translated text
Key words
spycatcher,lable protein,PD1,PDL1
AI Read Science
Must-Reading Tree
Example
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined