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TNF-α诱导A549细胞中NF-κB与NOX2的相关性研究

Weijing WU,Jiamin ZHANG,Liping LI

Journal of Clinical Emergency（2017）

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Abstract
目的:观察TNF-α刺激前后,NOX2基因在肺泡Ⅱ型上皮A549细胞的表达情况;探讨NF-κB与NOX2基因的相关性.方法:预转染NF-κB siNRA,以TNF-α刺激肺泡Ⅱ型上皮A549细胞,采用RT-PCR及免疫印迹法分别检测NOX2mRNA及蛋白的表达水平.结果:以TNF-α刺激A549细胞可观察到NOX2mRNA及NOX2蛋白表达增加(P＜0.05);预转染NF-κB siRNA,NOX2mRNA及NOX2蛋白表达降低(P＜0.05).结论:TNF-α可诱导NOX2基因在A549细胞表达上调,预先沉默NF-κB可下调上述表达趋势,提示NFκB与NOX2基因表达存在相关性.
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Key words
acute lung injury,NF-κB,NOX2,oxidative stress
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