液相色谱串联质谱法和循环酶法测定血清中同型半胱氨酸的相关性
Chinese Journal of Clinical Laboratory Science（2017）
Abstract
目的 分析液相色谱串联质谱(LC-MS/MS)法与循环酶法测定人血清中同型半胱氨酸(homocysteine,HCY)浓度的相关性.方法 收集63例患者的血清样本,用LC-MS/MS法和循环酶法分别测定相同样本的HCY浓度,并评价2种方法的相关性.结果 LC-MS/MS法与循环酶法对HCY浓度测定的结果分别为(19.11±15.69) μmol/L和(16.95±14.41) μmol/L,差异有统计学意义(t =6.25,P＜0.05).2种方法的线性回归方程为YLC-MS/MS法=1.074X循环酶法+0.892,相关系数(R) =0.987,相关性较好.结论 LC-MS/MS法与循环酶法测定血清中HCY浓度的相关性较好,LC-MS/MS法适用于临床对HCY的检测.
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Key words
homocysteine,LC-MS/MS method,enzymatic cycling assay,correlation
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