特敏增强荧光染色法在浅部真菌病诊断中的应用
Chinese Journal of Clinical Laboratory Science（2017）
Abstract
目的 评价特敏增强荧光染色法在浅部真菌病诊断中的应用价值.方法 对200例临床高度怀疑真菌感染的标本分别用特敏增强荧光染色法、钙白荧光染色法和传统的1.78 mol/L KOH湿片法对真菌进行镜下形态学观察,并比较真菌检出率.结果 特敏增强荧光染色法视野背景干净,菌丝及孢子均为亮蓝色,真菌的形态结构清晰易辨.特敏增强荧光染色法检出率高于钙白荧光染色法和KOH湿片法(x2=17.984,P＜0.05;x2 =32.063,P＜0.05).结论 荧光染色法是一种快速有效的真菌染色镜检技术,在临床实验室早期诊断真菌病中有推广价值.
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Key words
hypersensitive,enhanced fluorescent staining,fungal direct microcopic examination,potassium hydroxide based smear assay,calcofluor white staining
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