改良的甲基化特异性多聚酶链反应检测肝细胞癌基因甲基化
CHINESE JOURNAL OF DIGESTION(2004)
Abstract
甲基化特异PCR(methylation-specific PCR,MSP)技术利用了PCR灵敏、快速、简便的特点,已经成为当前筛选DNA甲基化的常用方法之一.但该方法操作繁琐,化学修饰后的DNA质量差,不易保存和PCR扩增[1],有待进一步改进.
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