Chrome Extension
WeChat Mini Program
Use on ChatGLM

ADAM17调控CGRP对血管紧张素Ⅱ诱导的血管平滑肌细胞增殖的抑制效应

Chinese Journal of Arteriosclerosis(2017)

Cited 0|Views6
No score
Abstract
目的 阐明肿瘤坏死因子α转化酶(ADAM17)调控降钙素基因相关肽(CGRP)的抗血管平滑肌细胞(VSMC)增殖效应.方法 以大鼠源性胸主动脉平滑肌细胞株(A10VSMC)为研究对象,采用RNA干扰抑制AD-AM17表达;采用蛋白质免疫印迹和实时定量PCR检测蛋白和mRNA表达水平;采用噻唑蓝比色法评估细胞活性.结果 CGRP显著抑制血管紧张素Ⅱ介导的VSMC增殖.血管紧张素Ⅱ刺激24 h后,VSMC中ADAM17表达显著增加.CGRP预处理30 min能显著降低VSMC中ADAM17表达,而CGRP受体拮抗剂CGRP8-37可抑制或逆转上述效应.ADAM17 RNA干扰可显著抑制血管紧张素Ⅱ介导的VSMC增殖.结论 降低ADAM17表达可能是CGRP抑制血管紧张素Ⅱ介导的VSMC增殖效应的机制.
More
Translated text
Key words
A disintergrin and metalloproteinase 17,Calcitonin gene-related peptide,Cell proliferation,Vascular smooth muscle cell,Angiotensin Ⅱ
AI Read Science
Must-Reading Tree
Example
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined