羟基红花黄色素A抑制转化生长因子-β1诱导NIH/3T3细胞的活化作用
Journal of Cardiovascular and Pulmonary Diseases(2015)
Abstract
目的:探讨羟基红花黄色素A(HSYA)抑制转化生长因子β1(TGF-β1)诱导NIH/3T3细胞活化的作用.方法:NIH/3T3细胞分8组:正常对照组、HSYA对照组、TGF-β1组、TGF-β1+0.5μmol/L HSYA组、TGF-β1+1μmol/L HSYA组、TGF-β1+ 2μmol/L HSYA组、TGF-1+ 2μmol/L SB-431542和TGF-β1+ SB-431542 +2μmol/L HSYA组.采用四甲基偶氮唑蓝(MTT)法检测细胞的增殖;细胞划痕实验观察细胞的迁移.结果:MTT法观察结果表明:与各时间点正常对照组相比,TGF-β1作用24,48及72小时后的OD 570nm值明显升高(均P<0.01),并且同时高于TGF-β1+HSYA各组(均P<0.05)和TGF-β1+ SB-43154两组.在TGF-β1作用0,2,8,12及24小时后,与正常对照组相比,细胞迁移距离明显增加(均P<0.001),并且同时高于TGF-β1+ HSYA组(均P<0.001)和SB-431542组.结论:HSYA可抑制TGF-β1诱导NIH/3T3细胞活化作用并且呈现时效关系和量效关系.
MoreKey words
Hydroxysafflor yellow A,TGF-β1,fibroblast,cell proliferation,cell migration
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