胃泌素释放肽对肝癌细胞MHCC97H迁移的作用
Chinese Journal of General Surgery(2012)
Abstract
目的:探讨胃泌素释放肽(GRP)对高转移人肝癌细胞MHCC97H迁移和上皮间质转化(EMT)的作用.方法:利用外源性的GRP刺激MHCC97H细胞,细胞三维培养观察细胞形态的变化,Western blot检测EMT标志蛋白E-cadherin和vimentin的表达,Transwell迁移分析检测肝癌细胞的迁移数量的变化.结果:与对照组相比较,GRP刺激的人高转移肝癌细胞MHCC97H向间叶细胞的形态转变,使上皮细胞表型标记物E-cadherin蛋白表达下降,间叶细胞表型标记物vimentin蛋白表达升高,细胞迁移数量明显增多.其作用能被GRP受体阻滞剂PD176252所减弱.结论:GRP能够诱导人高转移肝癌细胞MHCC97H发生EMT,促进肝癌细胞的迁移,该作用是通过GRP结合GRPR实现的.
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