miR-337-3p对胃癌细胞SGC7901中HPSE表达的调控及其对细胞增殖的影响
Chinese Journal of Current Advances in General Surgery（2019）
Abstract
目的:探讨miR-337-3p对胃癌细胞SGC7901中HPSE表达的调控作用及其对细胞增殖的影响.方法:miR-337-3p mimics转染SGC7901细胞后,应用RT-PCR、Western blot检测其对miR-337-3p表达水平的影响及其对HPSE表达的影响;荧光素酶实验检测转染细胞中HPSE 3'-UTR荧光素酶活性;CCK-8测定细胞增殖的影响.结果:上调miR-337-3p可以抑制HPSE的表达、降低HPSE 3'-UTR启动子荧光素酶活性,并可抑制胃癌细胞增殖.结论:miR-337-3p可以通过下调HPSE的表达抑制胃癌细胞SGC7901增殖.
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