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HPLC-ELSD法测定黄芪中黄芪甲苷的含量

张雪梅,屈巧玲

JOURNAL OF PEDIATRIC PHARMACY（2004）

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Abstract
目的:建立HPLC-ELSD法测定黄芪中黄芪甲苷的含量.方法:采用高效液相色谱法蒸发光散射检测器测定,Phenomenex公司Luna C18色谱柱(5μm,150 mm×4.6 mm),流动相为甲醇-水(75:25);流速:1.0 ml/min;ELSD参数:漂移管温度40℃;氮气压力:2.0 Bar.结果:平均回收率为98.23%,RSD=1.87%.结论:该方法对黄芪甲苷的测定良好.
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