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促红细胞生成素对大鼠脑缺血再灌注后claudin-5和TNF-α表达的影响

刘坤,姚阳,商丽宏,徐畅,薛一雪

Journal of Apoplexy and Nervous Diseases（2015）

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Abstract
目的 探讨促红细胞生成素(erythropoietin,EPO)对大鼠脑缺血再灌注后紧密连接(tight junctions,TJ)蛋白claudin-5和肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)的影响.方法 采用大鼠大脑中动脉栓塞(MCAO)模型,伊文思蓝渗透性实验检测血脑屏障(blood brain barrier,BBB)通透性,透射电镜观察脑微血管内皮细胞间TJ的状态,免疫组化法测定claudin-5的蛋白表达,ELISA法检测TNF-α的含量.结果 EPO能够显著降低脑缺血再灌注后BBB的通透性(P＜0.01),恢复TJ完整性,显著上调缺血脑组织中claudin-5表达水平(P＜0.01),显著降低TNF-α含量.结论 EPO通过降低脑缺血再灌注后脑组织中TNF-α含量,使claudin-5的表达增加,从而恢复TJ完整性,降低BBB通透性.
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Key words
Cerebral ischemia reperfusion,Blood brain barrier,Erythropoietin,Claudin-5,TNF-α
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