SAHA对骨癌痛大鼠脊髓背角内谷氨酸转运体1表达的影响
Chinese Journal of Neuroanatomy(2017)
Abstract
目的:通过观察辛二酰苯胺异羟肟酸(Suberoylanilide Hydroxamic Acid,SAHA)对于骨癌痛(cancer-induced bone pain,CIBP)大鼠脊髓内谷氨酸转运体1(glutamate transporter 1,GLT-1)表达的影响,探讨GLT-1参与SAHA镇痛的相关机制.方法:将体重180~220 g雌性SD大鼠随机分为4组:Sham+ vehicle组(A)、Sham+SAHA组(B)、CIBP+vehicle组(C)、CIBP+ SAHA组(D).经腹膜腔注射50 mg/kg SAHA,1/d.利用von Frey 纤维测量大鼠手术侧机械缩足反射阈值(paw withdrawal threshod,PWT).上述4组大鼠在不同时间点处死后取其腰膨大段脊髓,应用免疫组织化学染色和Western Blot方法观察大鼠腰膨大节段手术侧脊髓背角内GLT-1的表达情况.结果:与A组相比,C组大鼠PWT自术后第7d开始显著下降,且一直持续至术后第14 d(P<0.01),脊髓背角内GLT-1水平逐渐下调,呈时间依赖性(P<0.05).D组与C组相比,PWT升高(P<0.05),术侧脊髓背角内GLT-1表达增加(P<0.05).结论:腹膜腔注射SAHA能够有效缓解骨癌痛,其部分机制可能与促进脊髓背角内GLT-1的表达有关.
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Key words
cancer-induced bone pain,GLT-1,SAHA,rat
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