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富自体浓缩生长因子纤维蛋白提取液对MC3T3-E1成骨细胞增殖与分化的影响

Zhong-yan ZHU,Lu ZHANG,Min LIU

Journal of Oral Science Research（2017）

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Abstract
目的:提取富自体浓缩生长因子纤维蛋白(concentrated growth factors,CGF),研究CGF提取液对MC3T3-E1成骨细胞体外增殖、分化能力的影响.方法:制取CGF提取液,实验组采用CGF条件培养基,对照组为α-MEM完全培养基分别培养MC3T3-E1细胞,CCK-8法测定细胞增殖情况;ALP检测碱性磷酸酶活性;RT-PCR测定Runt相关转录因子-2(Runx2)和成骨细胞特异性转录因子(Osx)基因表达情况.结果:CCK-8显示各浓度CGF提取液有不同程度促增殖作用,其中10％CGF强于其他组;以10％CGF为实验浓度,ALP活性检测显示实验组3、5、7d活性增强;RT-PCR结果显示实验组Runx2和Osx的基因表达水平均明显增高.结论:CGF释放的生长因子可促进MC3T3-E1成骨细胞增殖、分化.
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Key words
Concentrated growth factors (CGF),Osteoblasts,Proliferation,Differentiation
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